Translational_Unit

Part:BBa_K152001:Design

Designed by: David Johnston Monje   Group: iGEM08_Guelph   (2008-10-29)


rbs + crtE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 732
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted to use this in synthetic operon construction, and as the first gene in the transcript, so we included a NdeI restriction site at the start codon.


Source

The plasmid pAC-3.10.10.A which was sent to us by Ethan Johnson on iGEM Minnesota team timebomb.


References