Translational_Unit
Part:BBa_K152001:Design
Designed by: David Johnston Monje Group: iGEM08_Guelph (2008-10-29)
rbs + crtE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 732
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We wanted to use this in synthetic operon construction, and as the first gene in the transcript, so we included a NdeI restriction site at the start codon.
Source
The plasmid pAC-3.10.10.A which was sent to us by Ethan Johnson on iGEM Minnesota team timebomb.